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Open Access Research article

Potent α-glucosidase and α-amylase inhibitory activities of standardized 50% ethanolic extracts and sinensetin from Orthosiphon stamineus Benth as anti-diabetic mechanism

Elsnoussi Ali Hussin Mohamed1*, Mohammad Jamshed Ahmad Siddiqui2, Lee Fung Ang1, Amirin Sadikun1, Sue Hay Chan1, Soo Choon Tan1, Mohd Zaini Asmawi1 and Mun Fei Yam1*

Author Affiliations

1 School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang 11800, Malaysia

2 Kulliayya of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang 25200, Malaysia

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BMC Complementary and Alternative Medicine 2012, 12:176  doi:10.1186/1472-6882-12-176

Published: 8 October 2012

Abstract

Background

In the present study, we tested a 50% ethanolic extract of Orthosiphon stamineus plants and its isolated bioactive compound with respect to their α-glucosidase and α-amylase inhibitory activities.

Methods

Bioactive flavonoid sinensetin was isolated from 50% ethanolic extract of Orthosiphon stamineus. The structure of this pure compound was determined on the NMR data and the α-glucosidase and α-amylase inhibitory activities of isolated sinensetin and 50% ethanolic extract of Orthosiphon stamineus were evaluated.

Results

In vitro studies of a 50% ethanolic extract of O. stamineus and the isolated sinensetin compound showed inhibitory activity on α-glucosidase (IC50: 4.63 and 0.66 mg/ml, respectively) and α-amylase (IC50: 36.70 mg/ml and 1.13 mg/ml, respectively). Inhibition of these enzymes provides a strong biochemical basis for the management of type 2 diabetes via the control of glucose absorption.

Conclusion

Alpha-glucosidase and α-amylase inhibition could the mechanisms through which the 50% ethanolic extract of O. stamineus and sinensetin exert their antidiabetic activity, indicating that it could have potential use in the management of non-insulin-dependent diabetes.