Effects of EOFAZ on trypan blue exclusion staining in HUVECs' injury induced by ox-LDL. HUVECs were pretreated for 30 min with EOFAZ or PRA, and then exposed to 100 mg/L ox-LDL for 24 h. A cell suspension was mixed with a 0.4% trypan blue dye for 5 min at 25°C, and the unstained (viable) and stained (nonviable) cells were counted in a hemacytometer. The values shown are mean ± S.E.M. of three experiments (four or five cultures per experiment). ##p < 0.01, vs. control group; *p < 0.05, **p < 0.01, vs. ox-LDL group.
Shen et al. BMC Complementary and Alternative Medicine 2012 12:174 doi:10.1186/1472-6882-12-174