Identification of eckol and 6,6’-bieckol in EMM. (A) HPLC chromatogram of EMM and the chemical structures of eckol and 6,6’-bieckol (insert). (B) Cells pretreated with different concentrations of eckol or 6,6’-bieckol for 1 h were stimulated with LPS for 16 h. The expression levels of iNOS and COX-2 in cells were analyzed by Western blotting using corresponding antibodies. Relative density ratios of each protein over β-actin are shown below the blots. The results presented are representative of three independent experiments. ap < 0.05 indicates significant differences compared to the control group. bp < 0.05 indicates significant differences compared to the LPS-only treated group.
Joung et al. BMC Complementary and Alternative Medicine 2012 12:171 doi:10.1186/1472-6882-12-171