Topical application of water soluble extract of P. linteus improves the symptoms of experimental atopic dermatitis. (A) Experimental AD was induced on the both ears of BALB/c mice by alternative painting of DNCB (0.2 mg/each ear) and mite extract (0.2 mg/each ear) for 2 weeks. After 2 weeks of AD induction, control PBS (Cont) or water soluble extract of P. linteus (WA, 1 mg/each ear) or ceramide (Cera, 15 μg/each ear) were painted daily for 2 weeks while continuously inducing experimental atopic dermatitis. (B) Representative mice of each treatment group at day 14 and day 30 are shown. During AD induction period, ear thickness (C) and clinical score (D) was measured 24 hrs after application of DNCB or mite extract. (E) Infiltrated lymphocytes into ears were observed by H&E staining. Error bars indicated S.D. One (*), two (**) and three asterisk (***) indicates P < 0.05, P < 0.005, P < 0.001, respectively. Data are representative of three independent experiments. Without AD induction (normal health mice) is indicated as “W/O”.
Hwang et al. BMC Complementary and Alternative Medicine 2012 12:159 doi:10.1186/1472-6882-12-159