KT-NB does not affect Aurora A localization and histone H3 phosphorylation on serine10. HeLa cells were treated with 6.75 μg/ml of KT-NB for 24 h and harvested for immunofluorescence analysis. DAPI was used for DNA staining, while anti-tubulin shows the mitotic spindle. Cells were immunostained with. anti-Aurora A (A) or Anti-phospho-histone H3 (Ser10) (B). Merged images DAPI is blue, anti-tubulin is red, Aurora A (A) or histone H3 (B) is green. Scale bar: 5 μm.
Hsieh et al. BMC Complementary and Alternative Medicine 2012 12:149 doi:10.1186/1472-6882-12-149