Molecular mechanisms of GLY on FBS-induced VSMCs. (A). Effects of GLY on TIMP-1, TIMP-2, MMP-2, MMP-9 proteins expression. VSMCs were treated with 0.125, 0.25 and 0.5 mg/mL for 24 h, and cell lysates were subjected to SDS-PAGE followed by Western blotting. (B). Dose-dependent effects of GLY on the protein expression level of FAK, the phosphorylated FAK, ERK1/2, the phosphorylated ERK1/2, PI3K, MMP-2, MMP-9, AKT and the phosphorylated AKT. VSMCs were treated with 0.125, 0.25 and 0.5 mg/mL of GLY for 24 h. The expression of these proteins were analyzed by Western blotting. β-actin was used as a loading control.
Chien et al. BMC Complementary and Alternative Medicine 2012 12:137 doi:10.1186/1472-6882-12-137