Effects of GLY on cell viability and cell cycle analysis. (A). Effects of GLY on cell growth of VSMCs by MTT assay. The cells were incubated for 24 h with 10% fetal bovine serum alone (control) or with different concentrations of GLY (0.0625, 0.125, 0.25, 0.5, 1 and 2 mg/mL). (B). Flow cytometric analysis of GLY on the cell cycle of VSMCs cells. All the cells were treated with 15% fetal bovine serum with the addition of GLY at 0.0625,0.125,0.25,0.5,1 mg/mL for 18 h. (C). The percentage of subG1 under flow cytometric analysis. Values are means of three separate experiments, with standard errors represented by vertical bars. Mean value was significantly different from that of the control group :* p < 0.05,** p < 0.01, *** p < 0.001.
Chien et al. BMC Complementary and Alternative Medicine 2012 12:137 doi:10.1186/1472-6882-12-137