A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways
1 Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 402, Taiwan
2 School of Pharmacy, China Medical University, 91 Hsueh-Shih Road, Taichung, 404, Taiwan
BMC Complementary and Alternative Medicine 2012, 12:137 doi:10.1186/1472-6882-12-137Published: 24 August 2012
This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.
VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.
Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.
These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.