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Open Access Research article

A Chinese herbal formula "Gan-Lu-Yin" suppresses vascular smooth muscle cell migration by inhibiting matrix metalloproteinase-2/9 through the PI3K/AKT and ERK signaling pathways

Yi-Chung Chien1, Ming-Jyh Sheu2*, Chieh-Hsi Wu2, Wen-Hsin Lin2, Ying-Yi Chen2, Po-Liang Cheng1 and Hsu-Chen Cheng1*

Author Affiliations

1 Department of Life Sciences, and Agricultural Biotechnology Center, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 402, Taiwan

2 School of Pharmacy, China Medical University, 91 Hsueh-Shih Road, Taichung, 404, Taiwan

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BMC Complementary and Alternative Medicine 2012, 12:137  doi:10.1186/1472-6882-12-137

Published: 24 August 2012

Abstract

Background

This study was to explore the effects of Gan-Lu-Yin (GLY) on the migration of vascular smooth muscle cells (VSMCs) induced by fetal bovine serum and on neointima formation in a rat model of carotid artery balloon injury.

Methods

VSMCs were treated with different concentrations of GLY, and then analyzed with Flow cytometric analysis, zymography, transwell, and western blotting. SD rats received balloon-injury were analyzed with H&E staining.

Results

Our results showed that GLY significantly decreased the thickness of neointima. The inhibition by non-cytoxic doses of GLY of VSMCs migration was through its negative regulatory effects on phosphorylated ERK1/2, PI3K/AKT, and FAK. The data showed that GLY can inhibit the migration of VSMCs cells, and might block injury-induced neointima hyperplasia via the inhibition of VSMCs migration, without inducing apoptosis.

Conclusions

These observations provide a mechanism of GLY in attenuating cell migration, thus as a potential intervention for restenosis.

Keywords:
Chinese herbs; Gan-Lu-Yin; Migration; Restenosis; Vascular smooth muscle cell