Effects of the isolated compounds on the expression of COX-2 and iNOS mRNA in J774 cells. Cells seed in 100-mm dishes were treated with benzyl alcohol (78.6 nM), hydrobenzoin (2.27 μM), and 5-HMF (0.99 μM) respectively for 24 h, (-) means control group and (+) means add sample. Total RNA (3 μg) extracted with isogen reagents was subjected to RT-PCR for the amplification of COX-2 (a), iNOS (b), GAPDH (c) mRNA as described in the text. COX-2 and iNOS mRNA signal intensity was normalized to GAPDH mRNA expression in same sample.
Zhou et al. BMC Complementary and Alternative Medicine 2012 12:12 doi:10.1186/1472-6882-12-12