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Open Access Research article

Black tea extract prevents lipopolysaccharide-induced NF-κB signaling and attenuates dextran sulfate sodium-induced experimental colitis

Young-A Song1, Young-Lan Park1, Kyu-Yeol Kim1, Cho-Yun Chung1, Gi-Hoon Lee1, Dae-Ho Cho1, Ho-Seok Ki1, Kang-Jin Park1, Sung-Bum Cho1, Wan-Sik Lee1, Nacksung Kim2, Bong-Whan Ahn3 and Young-Eun Joo1*

Author Affiliations

1 Department of Internal Medicine, Chonnam National University Medical School, Gwangju, Korea

2 Department of Pharmacology, Chonnam National University Medical School, Gwangju, Korea

3 Department of Biochemistry, Chonnam National University Medical School, Gwangju, Korea

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BMC Complementary and Alternative Medicine 2011, 11:91  doi:10.1186/1472-6882-11-91

Published: 11 October 2011

Abstract

Background

Black tea has been shown to elicit anti-oxidant, anti-carcinogenic, anti-inflammatory and anti-mutagenic properties. In this study, we investigated the impact of black tea extract (BTE) on lipopolysaccharide (LPS)-induced NF-κB signaling in bone marrow derived-macrophages (BMM) and determined the therapeutic efficacy of this extract on colon inflammation.

Methods

The effect of BTE on LPS-induced NF-κB signaling and pro-inflammatory gene expression was evaluated by RT-PCR, Western blotting, immunofluorescence and electrophoretic mobility shift assay (EMSA). The in vivo efficacy of BTE was assessed in mice with 3% dextran sulfate sodium (DSS)-induced colitis. The severity of colitis was measured by weight loss, colon length and histologic scores.

Results

LPS-induced IL-12p40, IL-23p19, IL-6 and IL-1β mRNA expressions were inhibited by BTE. LPS-induced IκBα phosphorylation/degradation and nuclear translocation of NF-κB/p65 were blocked by BTE. BTE treatment blocked LPS-induced DNA-binding activity of NF-κB. BTE-fed, DSS-exposed mice showed the less weight loss, longer colon length and lower histologic score compared to control diet-fed, DSS-exposed mice. DSS-induced IκBα phosphorylation/degradation and phosphorylation of NF-κB/p65 were blocked by BTE. An increase of cleaved caspase-3 and poly (ADP-ribose) polymerase (PARP) in DSS-exposed mice was blocked by BTE.

Conclusions

These results indicate that BTE attenuates colon inflammation through the blockage of NF-κB signaling and apoptosis in DSS-induced experimental colitis model.

Keywords:
Black tea; NF-κB; Macrophage; Dextran sulfate sodium; Colon inflammation