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Open Access Research article

Anti-inflammatory and antioxidant effects of Tualang honey in alkali injury on the eyes of rabbits: Experimental animal study

Karuppannan Bashkaran, Embong Zunaina*, Shaharuddin Bakiah, Siti Amrah Sulaiman, KNS Sirajudeen and Venkatesh Naik

BMC Complementary and Alternative Medicine 2011, 11:90  doi:10.1186/1472-6882-11-90

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Test protocol

Carol Granger   (2011-11-03 12:02)  The Granger Partnership email

I was dissappointed to read the technique used in this study, and even more so when I read the authors' call for more rabbits to use to repeat in a larger study.
There are a range of other assays that can be used to assess anti-inflammatory activity of substances. These include ex vivo corneas and other tissue culture methods. As scientists, we should all be trying to work towards the three R's: Refine, Reduce, Replace. We shouldn't be doing experiments just because we can, but rather should be doing good meaningful science without unnecessary suffering.

Competing interests

I declare I have no competing interests. I am a clinical nutrition practitioner and post-graduate researcher with qualifications in biochemistry and in microbiology. I have previously worked in a human tissue bank where ethically consented, non-transplantable human tissues were made available for research purposes.


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