Figure 4.

Cell proliferation, dendritic maturation and synaptic plasticity in the dentate gyrus of the mice hippocampus. (A) The number of BrdU+ cells decreased after exogenous corticosterone administration; this number significantly increased after Hypericum perforatum treatment. Values plotted are mean ± SEM (N = 8 per group). * p < 0.05, ° p < 0.05, versus control group and corticosterone/vehicle group, respectively. (B) BrdU of vehicle-treated groups at 40X magnification (B') BrdU of vehicle-treated groups at 100X magnification (C) BrdU of Hypericum perforatum at 40X magnification (C') BrdU of Hypericum perforatum at 100X magnification (D) The total number of DCX+ cells did not change after corticosterone administration. Hypericum perforatum treatment increased the number of DCX+ cells in corticosterone treated mice. Values plotted are mean ± SEM (N = 8 per group). ° p < 0.05, versus corticosterone/vehicle group. (E) DCX of vehicle-treated groups at 40X magnification (E') DCX of vehicle-treated groups at 100X magnification (F) DCX of Hypericum perforatum at 40X magnification (F') BrdU of Hypericum perforatum at 100X magnification (G) The number of spines in DG revealed a significant reduction in the development of dendritic spines in cort+ mice that was reverted by Hypericum perforatum treatment. Values plotted are mean ± SEM (N = 8 per group). * p < 0.05, ° p < 0.05, § p < 0.05 versus vehicle group, corticosterone/vehicle group, and Hypericum perforatum/vehicle group, respectively. (H) Photomicrograph illustrating the apical dendrites of granular cells in cort-/Hyp- group (I) Photomicrograph illustrating the apical dendrites of granular cells in cort+/Hyp+ group

Crupi et al. BMC Complementary and Alternative Medicine 2011 11:7   doi:10.1186/1472-6882-11-7
Download authors' original image