Table 2

Expression of PPARγ, COX-2 and iNOS in human HCC cell lines

PPARγ expression

COX-2 expression

iNOS expression




IGF-1

L.obtusiloba extract

IGF-1 and L.obtusiloba extract

IGF-1

L.obtusiloba extract

IGF-1 and L.obtusiloba extract

IGF-1

L.obtusiloba extract

IGF-1 and L.obtusiloba extract


HepG2

4.31 ± 0.51*

0.76 ± 0.14

1.15 ± 0.09#

n.d.

n.d.

n.d.

1.17 ± 0.07

0.21 ± 0.14*

0.21 ± 0.09*;#

Hep3B

1.33 ± 0.12*

0.84 ± 0.09

0.81 ± 0.05#

2.28 ± 0.19*

0.77 ± 0.08*

1.09 ± 0.04*;#

n.d.

n.d.

n.d.

Huh-7

1.17 ± 0.05

0.21 ± 0.10*

0.31 ± 0.12*;#

n.d.

n.d.

n.d.

n.d.

n.d.

n.d.

SK-Hep1

1.43 ± 0.11*

0.75 ± 0.09*

0.89 ± 0.07#

3.21 ± 0.34*

0.80 ± 0.07*

0.82 ± 0.09#

1.87 ± 0.12*

0.27 ± 0.12*

0.35 ± 0.06*;#


Cell lysates of HCC cell lines as described for Table 1, were subjected to specific western-blots for PPARγ, COX 2 and iNOS and for β actin as equal loading control. Densitometry of specific band intensity was normalized to β-actin expression. Mean values ± SD from three independent experiments. PPARγ, COX-2 and iNOS protein expression levels were calculated in relation to the respective untreated cells. Mean values ± SD from three independent experiments. *P < 0.05 compared to the untreated control, # P < 0.05 compared to IGF-1-treated cells, n.d. - not detected

Freise et al. BMC Complementary and Alternative Medicine 2011 11:39   doi:10.1186/1472-6882-11-39

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