Table 1

Expression of VEGF and HIF-1α in human HCC cell lines

VEGF expression

HIF-1α expression



IGF-1

L.obtusiloba extract

IGF-1 and L.obtusiloba extract

IGF-1

L.obtusiloba extract

IGF-1 and L.obtusiloba extract


HepG2

1.02 ± 0.03

0.75 ± 0.10*

0.93 ± 0.06

3.58 ± 0.26*

0.72 ± 0.07*

0.82 ± 0.11#

Hep3B

0.82 ± 0.18

0.67 ± 0.09*

0.54 ± 0.10*,#

1.52 ± 0.21*

0.62 ± 0.11*

0.63 ± 0.07*,#

Huh-7

1.38 ± 0.05*

0.28 ± 0.10*

0.47 ± 0.08*,#

0.89 ± 0.12

0.14 ± 0.04*

0.05 ± 0.03*,#

SK-Hep1

3.28 ± 0.24*

0.93 ± 0.10

0.83 ± 0.09#

1.28 ± 0.13*

0.67 ± 0.09*

0.68 ± 0.12*,#


Whole cell lysates from cells treated with 100 μg/ml L.obtusiloba extract, 125 ng/ml human IGF-1 or a combination of both for 48 h and from untreated cells as control were analyzed by western-blot specific for VEGF and HIF-1α. β-Actin was stained for equal loading control and specific band intensities were normalized to β-actin. VEGF and HIF-1α protein expression levels were calculated in relation to the respective untreated cells. Mean values ± SD from three independent experiments. *P < 0.05 compared to the untreated control, # P < 0.05 compared to IGF-1-treated cells.

Freise et al. BMC Complementary and Alternative Medicine 2011 11:39   doi:10.1186/1472-6882-11-39

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