Quantitative analysis of Boswellia sacra essential oil-induced human breast cancer cell death. Human breast cell lines were seeded in each well of 96-well tissue culture plates at the concentration of 5x103 cells/well in triplicate. Following overnight adherence, cells were treated with either Boswellia sacra essential oil hydrodistilled at (A) 78 oC or (B) 100 oC. Cell death was determined at 3 hours following essential oil exposure by the LDH cytotoxicity detection kit. Data were presented as mean average numbers of dead cells ± SEM from at least 3 independent experiments. * indicates statistical differences of cell death between cancer cells and immortalized breast cells (P < 0.05).
Suhail et al. BMC Complementary and Alternative Medicine 2011 11:129 doi:10.1186/1472-6882-11-129