Images represent haemetoxylin and eosin stained pancreas sections of (A) control mice showing intact islet histoarchitecture, (B) diabetic mice showing islet cell destruction and wider intracellular spaces due to streptozotocin toxicity (C-E) diabetic animals treated with flavonoidal fraction at 100, 250 and 500 mg/kg bodyweight demonstrating ameliorating effects and improved islet integrity (Magnification 200×) (F) Quantification of islet cell apoptosis in multiple dose streptozotocin mouse treated with various concentrations of flavonoidal fraction (G) Images represent confocal optical slices of immunostained pancreas of control, diabetic and diabetic treated with various concentrations of flavonoidal fraction. Guinea Pig anti insulin (red) and ApoBrdu-dUTP (green) were used as primary antibodies. DAPI (Blue) was used to visualize nuclei. The slides were visualized by Laser Scanning Confocal Microscope (LSM 510 META, ZEISS, Germany). Scale bar represents 100 μm).
Ansarullah et al. BMC Complementary and Alternative Medicine 2011 11:126 doi:10.1186/1472-6882-11-126