Table 5

Effect of RHP and GLGPG on gene expression in human chondrosarcoma SW1353 cells

Gene

CTa

IL-1β

IL-1β + RHP (250 mg/L)

IL-1β + GLGPG (9.7 mg/L)

fold change

fold change

p valueb

fold change

p valueb


MMP-1

27.2

6.3

3.6

0.002

4.5

0.019

MMP-2

22.3

0.8

0.6

0.006

1.0

0.142

MMP-3

32.7

182.7

80.6

< 0.001

137.0

0.003

MMP-9

31.8

6.3

7.1

0.285

10.7

0.001

MMP-13

28.4

17.7

8.9

< 0.001

12.7

0.005

ADAMTS-4

35.3

10.7

9.8

0.136

9.6

0.026

ADAMTS-5

31.1

0.8

0.7

0.476

1.4

0.006

TIMP-1

21.2

1.4

1.2

0.195

1.9

0.007

Aggrecan

32.0

1.8

1.3

0.017

3.7

< 0.001

Collagen I

19.2

1.1

0.8

0.028

1.8

0.001

COL2A1

31.9

0.8

0.6

0.001

0.9

0.526

MIP-2

40.2

1169

474

< 0.001

1626

0.006

MIP-3α

38.5

19642

12001

< 0.001

20822

0.437

CCL5/RANTES

42.9

35920

27081

0.048

41476

0.353

IL-8

34.5

12022

5599

< 0.001

16308

0.001

CXCL10/IP-10

39.7

3274

836

0.001

1436

0.001

IL-1α

37.7

42.3

29.9

0.036

32.0

0.065

IL-1β

31.9

135.3

118.1

0.195

100.8

0.012

IL-6

38.4

10846

5969

0.001

8876

0.122

IL-1Ra

31.9

1.1

1.4

0.381

1.7

0.111

IL-1RI

27.1

1.7

0.9

0.001

1.7

0.885

TNF-α

34.5

319.2

705.1

0.001

567.8

0.003

iNOS

35.4

17.6

22.4

0.139

21.2

0.236

COX-2

34.4

23.4

19.0

0.080

35.3

0.005

LIF

30.1

64.8

53.9

0.118

42.1

0.005


IL-1β -stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 4 h and gene expression was quantified by RT-PCR. Fold changes were calculated as indicated in Materials and Methods. Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.

aCT = cycle threshold (CT < 22 = high basal gene expression; 23 < CT < 30 = intermediate basal gene expression; CT > 30 = low basal gene expression).

bp value = significance value between (IL-1β)- and (IL-1β + substance) -treatment.

Schwager et al. BMC Complementary and Alternative Medicine 2011 11:105   doi:10.1186/1472-6882-11-105

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