Table 4

Effect of RHP and GLGPG on gene expression in human PBL

Gene

CTa

LPS/IFN-γ

LPS/IFN-γ + RHP (250 mg/L)

LPS/IFN-γ + GLGPG (9.7 mg/L)

fold change

fold change

p valueb

fold change

p valueb


COX-2

22.8

8.6

8.6

0.880

6.8

0.16

TNF-α

24.9

16.1

8.9

0.004

12.4

0.04

IL-1α

27.2

17.9

11.3

0.002

16.1

0.37

IL-6

29.8

54.8

43.6

0.254

55.6

0.94

CCL5/RANTES

25.8

1.2

1.2

0.908

1.3

0.45

CXCL10/IP-10

35.5

1444

190

< 0.001

2340

0.04

MIP-2

28.6

16.8

18.7

0.577

15.6

0.66

MIP-3α

28.7

37.6

32.3

0.159

23.1

0.01

5-LOX

25.5

0.8

0.6

0.018

0.8

0.96

MMP-9

27.3

0.8

0.9

0.830

0.8

0.81


LPS/IFN-γ -stimulated cells were cultured with 31.3 - 1000 mg/L RHP or 1.2 - 38.7 mg/L of GLGPG for 2 h and gene expression was quantified by RT-PCR. Fold changes were calculated as indicated in Materials and Methods. Only the effects of 250 mg/L RHP and 9.7 mg/L GLGPG are shown.

aCT = cycle threshold (CT < 25 = high basal gene expression; 25 < CT < 30 = intermediate basal gene expression; CT > 30 = low basal gene expression).

bp value = significance value between (LPS/IFN-γ)- and (LPS/IFN-γ + substance) -treatment

Schwager et al. BMC Complementary and Alternative Medicine 2011 11:105   doi:10.1186/1472-6882-11-105

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