Table 1

Effect of RHP and GLGPG on NO and PGE2 production in RAW264.7 cells

Substance

IC50 ± SEM (NO) [mg/L]

IC50 ± SEM (PGE2) [mg/L]


GLGPGa

28.6 ± 4.6 (N = 6)

> 38.7c (N = 6)


RHPb

796.9 ± 36.9 (N = 15)

594 ± 43 (N = 14)


LPS-stimulated cells were cultured for 24 h with 31.3 - 1000 mg/L RHP, or 1.2 - 38.7 mg/L GLGPG. The concentration of NO and PGE2 was determined in culture supernatants and the IC50 values (± SEM, standard error of the mean) were computed. N, number of independent experimental series. At the conditions described in Materials and Methods, unstimulated and LPS-stimulated cells produced < 0.125 μM nitrite and 25.4 ± 1.2 μM, respectively.

aGLGPG: galactolipid (2S)-1, 2-di-O-[(9Z, 12Z, 15Z)-octadeca-9, 12, 15-trienoyl]-3-O-β-D-galactopyranosyl glycerol; MW 774

bFrom Hyben Vital

c Highest concentration of GLGPG tested. At 38.7 mg/L GLGPG, PGE2 production was inhibited by 14 ± 9%.

Schwager et al. BMC Complementary and Alternative Medicine 2011 11:105   doi:10.1186/1472-6882-11-105

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