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Open Access Research article

Rose hip and its constituent galactolipids confer cartilage protection by modulating cytokine, and chemokine expression

Joseph Schwager*, Ulrich Hoeller, Swen Wolfram and Nathalie Richard

Author Affiliations

DSM Nutritional Products Ltd., Department of Human Nutrition & Health, P.O. Box 2676, CH-4002 Basel, Switzerland

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BMC Complementary and Alternative Medicine 2011, 11:105  doi:10.1186/1472-6882-11-105

Published: 3 November 2011

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Additional file 1:

Effects of RHP and GLGPG on gene expression in murine macrophage cell line RAW264.7. RAW264.7 cells were stimulated with LPS and cultured with 250 mg/L RHP or 9.7 mg/L of GLGPG for 4 h and gene expression was quantified by RT-PCR. Fold changes were calculated as specified in Materials and Methods.

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Additional file 2:

Effects of RHP and GLGPG on cytokine/chemokine production in human peripheral blood leukocytes. LPS/IFN-γ -stimulated peripheral blood leukocytes were cultured with 250 mg/L RHP or 9.7 mg/L of GLGPG for 24 h and proteins were quantified by multi-parametric analysis as described in Materials and Methods.

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Additional file 3:

Effects of RHP and GLGPG on gene expression in human peripheral blood leukocytes. LPS/IFN-γ -stimulated cells were cultured with 250 mg/L RHP or 9.7 mg/L of GLGPG for 2 h and gene expression was quantified by RT-PCR (for details see: Materials and Methods).

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