Table 1

Oligonucleotide primer sequences used in RT-PCR

Target Gene

Primer sequence

Cycling condition

Size (bp)

PPAR γ2

Forward 5'-GCATGGTGCCTTCGCTGATGC-3'

Reverse 5'-AGGCCTGTTGTAGAGCTGGGT-3'

95°C/5 min

95°C/1 min,55°C/1 min

72°C/1 min,72°C/7 min

4°C hold, 33 cycles

340

SREBP-1c

Forward 5'-TTGCGCAAGGCCATCGACTACATT-3'

Reverse 5'-ACAAGGGGCTGCTCTGGAAAGGTG-3'

94°C/5 min

94°C/1 min,55°C/1 min

72°C/1 min,72°C/7 min

4°C hold, 26 cycles

238

Perilipin

Forward 5'-TGGAGACTGAGGAGAAGAAG-3'

Reverse 5'-ATGTGAGAGGGGAGATGG-3'

94°C/1.5 min

94°C/45 sec,52°C/1.5 min

72°C/1 min,72°C/7 min

4°C hold, 23 cycles

120

Resistin

Forward 5'-TGGTGGTGGTGGCTGTCCTGG-3'

Reverse 5'-GAGTGAGATGTGGTCTGGGCG-3'

95°C/2 min

95°C/45 sec,55°C/1.5 min

72°C/45 sec,72°C/7 min

4°C hold, 39 cycles

145

GAPDH

Forward 5'-AGTCAGCCGCATCTTCTTTTG-3'

Reverse 5'-CTCCTGGAAGGGAGATGG-3'

Condition adjusted as per the target gene

298

Nerurkar et al. BMC Complementary and Alternative Medicine 2010 10:34   doi:10.1186/1472-6882-10-34