Table 1

Primer sets and conditions for amplification of human KIT exons. Forward and reverse primers flanking each of the 21 exons are shown. The size of each exon-coding region, the size of each PCR product generated by each set of primers, and the respective annealing temperatures are indicated. Sequences marked by a ^* are derived from Spritz et al..[28]

Exon No

Exon # Size (bp)

Primers

PCR product Size (bp)

Annealing Tm(°C)

Forward Reverse

1

88(67)

5'GGACCAGAGCTCGGATC3' / 3'AGTCCTCTCTCCGGATG5'

198

62

2

270

5'CTCAGTATTGGAAGAAGTGC3' / 3'TATCTATGGCTCAGTCATCC5'

438

55

3

282

5'GCTTCTATAGATCCTGCC3' / 3'GATCAACGAGAAGAGAAGTC5' ^*

367

55

4

137

5'TGTACACATTTGAGGAGAAA3' ^* / 3'CTGACAGACGCACTAGTCG5' ^*

330

55

5

169

5'TGGAGAAGTTAATTGCTGCT3' ^* / 3'TGTGCTTTCATTGCAAGAGG5'

435

55

6

190

5'TTGTAATTCCAAGATGAGG3' ^* / 3'TACTAGGATGAGGACATAGG5'

422

55

7

116

5'TATGTGTGTGCGTGTTTATG3' ^* / 3'CAAGTTGAGTCCTTGCAGCTG5' ^*

372

62

8

115

5'CTCCTTGGTTCAGATTCTGC3' / 3'GTGAATTGCAGTCCTTCC5'

321

55

9

194

5'TATGCCACATCCCAAGTG3' / 3'GGTGTGATGCATGTATTACC5'

390

55

10

107

5'ACATAGCTTTGCATCCTGC3' / 3'ATTGTCTCAGTCATTAGAGCAC5' ^*

280

55

11

127

5'GAGTGCTCTAATGACTGAG3' / 3'CCACTGGAGTTCCTTAAAG5'

266

56

12

105

5'ATGGTCCTTCAATTCCACC3' / 3'TTCTGTCAAATGGGCACTC5'

270

55

13

111

5'GACATCAGTTTGCCAGTTG3' / 3'GCAAGAGAGAACAACAG5'

296

58

14

151

5'GACTAAGTAGTCTGATCC3' / 3'ACCCCATGAACTGCCTGTC5' ^*

327

55

15

92

5'TAGAGCATGACCCATGAG3' / 3'ACCCACTTGCAACCCTAACT5' ^*

325

60

16

128

5'GGTATGTCATTGCCACTG3' / 3'GGCTCTAAAATGCTCTGTTCT5' ^*

296

55

17

123

5'GTGAACATCATTCAAGGCG3' / 3'TTACATTATGAAAGTCACAGG5' ^*

390

55

18

112

5'CACATTTCAGCAACAGC3' / 3'CCTTCCTTGATCATCTTGT5' ^*

382

53

19

100

5'CTCAGAGCATCTTCTTGAAG3' / 3'ACATCTGGGTTTCTGTCTC5'

252

58

20

106

5'CCATATGTCCAGTTGCATAG3' / 3'TACCTGAAGCCCAATTTGC5'

257

60

21

2407(129)

5'TGGCCACAAAGTTCTTGG3' / 3'TATCCTGGAGTTGGATGC5'

369

55

Shibanuma et al. BMC Women's Health 2002 2:8   doi:10.1186/1472-6874-2-8