Mean melting profiles of perfect-match and mismatch probes using Alexa 594-labeled rRNA from Streptococcus mutans of six replicates in three experiments. Perfect-match (PM) probe and mismatch (MM) probe have Td (50% of signal remaining during analysis) values of 41.7°C and 34.0°C, respectively. The 95% confidence intervals are shown as dashed lines and MAXDCSD is 0.36 and occurs at 40°C. All values were calculated using fANOVA, see text for details (Bugli, submitted).
Starke et al. BMC Oral Health 2006 6(Suppl 1):S10 doi:10.1186/1472-6831-6-S1-S10