|Comparison of some staining principles in regard to their suitability for biofilm research|
|Staining principle||Stain(s) (combinations)||Comparison with microbiological data (PE)||Concentration independency||Suitability for biofilm research||Potentially mutagenic|
|Single species in vitro||Biofilm ex vivo or in situ|
|Vital fluorescence (FDA/EB) cf. [8,110,112]||Fluorescein diacetate, Ethidium bromide||+||++||Proven [8,110,112-115]||Proven (cf. Table 4)||+1|
|BacLight® (cf. Table 3)||Syto 9, Propidium iodide||-||-||Questionable (cf. Table 3)||Questionable (cf. Table 3)||+2|
|Staining according to [10,102]||Diverse substances||?||?||Non-existing or questionable||Non-existing or questionable||?|
2Generally to assume, partly proven.
?Partly known or not known or not available from the diverse substances.
Netuschil et al.
Netuschil et al. BMC Oral Health 2014 14:2 doi:10.1186/1472-6831-14-2