Table 1

MicroRNA Ct levels ± standard deviation of samples (n = 3)

miRNA

WAT preadipocytes

WAT adipocytes

BAT preadipocytes

BAT adipocytes


miR-21

23.2 ±0.4

22.3 ±0.2

23.9 ± 0.8

22.2 ±0.2

miR-24

25.5 ±0.5

25.2 ±0.1

25.5 ±0.7

24.9 ±0.3

miR-34c

28.8 ±0.1

28.7 ±0.4

29.0 ±1.2

27.1 ±0.2

miR-136

ND

ND

ND

ND

miR-143

31.6 ±0.6

29.4 ±0.6

31.8 ±1.2

29.3 ±0.5

miR-298

33.2 ±0.2

33.8 ±0.2

33.5 ±0.6

33.5 ±0.4

miR-346

35.7 ±1.1

36.4 ±0.8

35.7 ±0.6

34.7 ±0.4

miR-501

34.5 ±0.9

34.4 ±0.9

34.6 ±0.1

34.4 ±0.4

miR-718

ND

ND

ND

ND

miR-720

22.5 ±0.3

22.9 ±0.1

23.4 ±0.4

22.9 ±0.7


All miRNA assays are pre-developed assays (PDA) from Applied Biosystems. Reference RNAs used for normalisation, snoRNA142, did not show variation between samples. ND: Not detectable. Note that miRNA expression values where Ct = >>30 could indicate that the TaqMan assay is non-functional.

Keller et al. BMC Endocrine Disorders 2011 11:7   doi:10.1186/1472-6823-11-7

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