Binding of imatinib to NQO2. Binding of imatinib to NQO2 causes significant changes in the electronic absorption spectrum of the flavin cofactor. Shown is the spectrum of 18.3 μM NQO2 in the absence (solid line) and presence (dashed line) of 40 μM imatinib at 25°C. Inset, difference spectrum calculated by subtraction of the spectrum of the unbound protein from that of the imatinib-bound protein.
Winger et al. BMC Structural Biology 2009 9:7 doi:10.1186/1472-6807-9-7