Figure 3.

Structural comparison of raffinose- and sucrose-bound E342A with apo wild-type and E342A levansucrase. (A) Stereo diagram of the superimposition of apo wild-type (green), E342A (grey) and raffinose-bound E342A (pale red). (B) Superposition of sucrose-bound (1PT2, [11], light blue) and raffinose-bound E342A (this study). Dashed lines in cyan indicate H-bond interactions conserved between both complexes, those in red indicate additional contacts made by the galactosyl moiety.