Table 5

Spectrum of mutations in α-helicesa

Amino acid

Original residues

Expected residues

χ2

P value

Mutant residues

Expected residues

χ2

P value


A

89

83

0.45

5.03E-01

23

57

20.13***

7.25E-06

C

26

14

11.27***

7.88E-04

56

33

15.76***

7.18E-05

D

34

44

2.23

1.35E-01

59

38

11.78***

5.99E-04

E

61

96

12.71***

3.64E-04

26

33

1.54

2.15E-01

F

28

36

1.76

1.84E-01

37

38

0.02

8.87E-01

G

48

30

10.69***

1.08E-03

46

54

1.31

2.52E-01

H

23

21

0.13

7.19E-01

41

38

0.26

6.12E-01

I

41

51

1.96

1.61E-01

29

50

8.63**

3.30E-03

K

20

61

27.70***

1.41E-07

52

33

10.73***

1.05E-03

L

101

112

1.04

3.09E-01

49

78

10.86***

9.85E-04

M

41

29

5.11*

2.37E-02

24

21

0.34

5.59E-01

N

22

27

1.08

3.00E-01

29

38

2.08

1.49E-01

P

29

24

1.15

2.84E-01

90

57

19.38***

1.07E-05

Q

28

45

6.12*

1.34E-02

55

33

14.41***

1.47E-04

R

153

64

122.78***

1.56E-28

73

80

0.70

4.04E-01

S

36

46

2.22

1.36E-01

53

88

13.66***

2.19E-04

T

30

38

1.84

1.75E-01

61

57

0.31

5.79E-01

V

62

59

0.18

6.69E-01

69

57

2.61

1.06E-01

W

24

16

4.19*

4.06E-02

32

17

14.36***

1.51E-04

Y

32

33

0.01

9.29E-01

24

28

0.68

4.08E-01


Sum

928

928

928

928


aχ2-numbers in italics indicate underrepresentation and numbers in bold overrepresentation compared to random distribution based on amino acid frequencies. The results of the χ2 are shown with significance level: * P < 0.05; ** P < 0.01; *** P < 0.001.

Khan and Vihinen BMC Structural Biology 2007 7:56   doi:10.1186/1472-6807-7-56

Open Data