Table 1 |
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|
DNA cleavage and binding activities of wt R.Eco29kI and its mutants |
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|
R.Eco29kI |
DNA cleavage activity [% of wt] |
standard bimolecular binding model |
cooperative binding model |
|||||
|
|
||||||||
|
Kd, nM |
χ2 |
R2 |
Kd, nMn |
n |
χ2 |
R2 |
||
|
|
||||||||
|
WT |
100 |
16 ± 5 |
1500 |
0.78 |
299 ± 50 |
2.1 ± 0.2 |
360 |
0.95 |
|
Y49A |
0 |
49 ± 5 |
105 |
0.97 |
529 ± 50 |
1.9 ± 0.3 |
31 |
0.99 |
|
R86A |
100 |
21 ± 5 |
1232 |
0,82 |
344 ± 19 |
2.0 ± 0.4 |
415 |
0.95 |
|
R104A |
~2 |
43 ± 18 |
442 |
0.94 |
1911 ± 356 |
1.8 ± 0.2 |
27 |
0.99 |
|
H108F |
0 |
114 ± 25 |
1533 |
0.84 |
14285 ± 2000 |
2.0 ± 0.1 |
582 |
0.94 |
|
E142A |
0 |
45 ± 11 |
1480 |
0.87 |
970 ± 200 |
1.8 ± 0.4 |
547 |
0.95 |
|
N154L |
~10 |
50 ± 15 |
1267 |
0.75 |
3100 ± 700 |
2.0 ± 0.3 |
431 |
0.91 |
|
|
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|
Ibryashkina et al. BMC Structural Biology 2007 7:48 doi:10.1186/1472-6807-7-48 |
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