Model of human CK1α with NS5A substrate peptide (green) and ATP molecule (purple). a: Catalytic base Asp 136 in catalytic loop (cyan), NS5A peptide (green) containing the phosphoacceptor serine 232 and phosphoserine 229 are shown in stick representation. Glycine loop (blue) is also shown. b: Stereo figure showing the zoomed in view of the substrate interacting kinase residues Arg 214, Lys 260, Gly 251 (magenta) making ionic interactions with phosphoserine of NS5A peptide. Ser 230 of NS5A peptide interacts with Thr 212 by means of hydrogen bonding. Phospho acceptor serine 232 of NS5A is proximal to the γ phosphate of ATP molecule and the catalytic base Asp 136 to carry out phosphorylation.
Sudha et al. BMC Structural Biology 2012 12:28 doi:10.1186/1472-6807-12-28