Table 1 |
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|
Percentages of secondary structure of salmon and squid protamines in sperm nuclei. |
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|
Salmon protamine |
Squid protamine |
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|
|
|
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|
Assignment |
140 mM |
140 mM |
600 mM |
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|
|
|
|
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|
Band cm-1 |
% |
Band cm-1 |
% |
Band cm-1 |
% |
|
|
|
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|
Turns |
1674 |
16 |
1669 |
17 |
1669 |
17 |
|
Turns |
1663 |
22 |
1662 |
20 |
1661 |
22 |
|
α-helix |
1652 |
20 |
1650 |
40 |
1650 |
39 |
|
Random coil/flexible regions |
1642 |
42 |
1640 |
23 |
1640 |
22 |
|
Side Chain |
1609 |
- |
1609 |
- |
1609 |
- |
|
|
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|
The concentration of nuclei expressed as DNA concentration was 5 mg/ml. Band position (cm-1) and percentage area (%) and assignment of the components were obtained after curve fitting of the amide I band (D2O). The buffer was 10 mM Hepes plus 140 or 600 mM NaCl, pH7.0. The values were rounded off to the nearest integer. |
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|
Roque et al. BMC Structural Biology 2011 11:14 doi:10.1186/1472-6807-11-14 |
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