Table 1

Percentages of secondary structure of salmon and squid protamines in sperm nuclei.

Salmon protamine

Squid protamine



Assignment

140 mM

140 mM

600 mM




Band cm-1

%

Band cm-1

%

Band cm-1

%


Turns

1674

16

1669

17

1669

17

Turns

1663

22

1662

20

1661

22

α-helix

1652

20

1650

40

1650

39

Random coil/flexible regions

1642

42

1640

23

1640

22

Side Chain

1609

-

1609

-

1609

-


The concentration of nuclei expressed as DNA concentration was 5 mg/ml. Band position (cm-1) and percentage area (%) and assignment of the components were obtained after curve fitting of the amide I band (D2O). The buffer was 10 mM Hepes plus 140 or 600 mM NaCl, pH7.0. The values were rounded off to the nearest integer.

Roque et al. BMC Structural Biology 2011 11:14   doi:10.1186/1472-6807-11-14

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