Table 3

Summary of evaluation

Protein name

Partner protein name

PROFbval¹

POODLE-S²

FlexPred¹

Proposed method

Staphylococcus A

Human Fc fragment

×

○

×

○

Ran GTPase

RCC1

×

○

×

○

14-3-3

Serotonin N-acteylase

○

○

×

○

Actin

Profilin

×

×

×

×

Erythropoietin

EPO receptor

×

○

○

○

Fab fragment

Flu virus hemagglutinin

○

×

×

○

TGF-beta

TGF-beta receptor

×

○

○

○

Actin

Dnase I

×

×

×

×

Coagulation factor Vlla

Soluble tissue factor

○

×

×

○

Ran GTPase

Importin-beta

×

×

×

○

HPr kinase C-ter domain

HPr

○

○

○

○

HIV1 reverse transcriptase

Fab28

○

○

×

○

Ecotin

D102N trypsin

○

○

○

○

EPO receptor

Erythropoietin

×

×

×

○

Vitamin D bindings

Actin

○

×

○

○

Nitrogenase Fe

Nitrogenase Mo-Fe protein

○

×

×

○

CDK2 kinase

CDK inhibitor 3

×

×

×

○

Gelsolin

Actin

○

○

○

○

Importin-beta

Ran GTPase

○

×

×

○

Hirustatin

Kallikrein

×

×

×

×

'○' means that the predictor detects at least one overlap between the observed conformational change region and predicted high motion region. Conversely, '×' means that there is no overlap. PROFbval, POODLE-S, and FlexPred were performed with the default parameters; then candidate conformational change regions were determined as follows.

¹ Three or more consecutive residues predicted to be flexible.

² Disordered region longer than three residues.

Hirose et al. BMC Structural Biology 2010 10:20   doi:10.1186/1472-6807-10-20