Figure 6.

NcdT436S vs KIF1A. A, NcdT436S chain A (cyan) and B (green) differ in C-terminus orientation (chain A, magenta; chain B, pink), helix α4 differs in length and the neck helices show distinct trajectories as they leave the motor domain to form the coiled-coil stalk. B, Helix α4 is longer by two turns in KIF1A-ADP (dark cyan) than KIF1A-AMP·PNP (dark green; C-terminus, brown). C, Helix α6 is oriented similarly in NcdT436S chain A and KIF1A-ADP. D, The C-terminus of NcdT436S chain B and KIF1A-ATP (KIF1A-AMP·PNP) are similarly oriented and helix α4 is similar in length. See also Additional file 1 Figure S2.