Figure 1.

Bioluminescent C. elegans expressing the LUC+::GFP. A. Strain with luminescence array feIs5 in a glp-4(bn2) genetic background, displaying widespread in vivo luminescence. Image captured in complete darkness with 10 sec integration, after adding luminescence buffer plus 200 μM levamisole. Scale bar represents 100 μm. B. Image of a feIs5 worm shows that GFP expression is strongest in the pharynx and the tail areas. Widespread green fluorescence is shown by the glp-4(bn2); feIs5 strain.