Figure 7.

Transcripts and TREs over-represented in the detrusor inflamed when compared to mucosa inflamed (DIMI). The regulatory network was determined by a combination of SSH-selected transcripts (green) and PAINT 3.3 query of transcription factor database (TRANSFAC). PAINT 3.3 was employed to examine 2000 base pairs of regulatory region upstream of the transcriptional start site of each differentially expressed transcript detected with the SSH. Genbank accession numbers were used as the gene identifiers in PAINT test files. Individual elements of the matrix are colored by the significance p-values. Over-representation in the matrix when compared to the reference (all TFs in the PAINT database) is indicated in orange (0 < p < = 0.01) or green (0.01 < p < 0.05). For a detailed origin of each library please see Figure 1 and Material and methods.