Table 3

Gene-specific primers
Gene Primers 5—3 GenBank # (species) Amplicon size (bp) Source
18S S: GAG GTT CGA AGA CGA TCA GA BK000964 (many) 316 [65]
AS: TCG CTC CAC CAA CTA AGA AC
KCC1 S: GTT CGC CTC ACT CTT CCT GGC U55815 (rat) 419 [66]
AS: TGG GCC ACC ACA TAC AGG GA
KCC2 S: CAT CAC AGA TGA ATC TCG GG U55816 (rat) 213 [66]
AS: TTC TCT GGG TCT GTC TCC C
KCC3a S: CCT CGC CTC CTC ACC TTT GC AF211854 (mouse) 284 This study
AS: TCA CTC TGA CGC CAG CCA TTG
KCC3b S: AGT AAA AGC CCG GAT TCA GG AF211855 (mouse) 330 [66]
AS: ATG AAA GTA CCC ATT TGG GG
KCC4 S: AGG AAG CTG CTG AGC GCA C AF087436 (mouse) 443 [66]
AS: CAG CAT TGT ACA GGT GCA GC
WNK1 S: ACC AGA AAG CCT CAT GTA AGC C NM_053794 (rat) 301 This study
AS: GTC CGC AGG GAA CGT CAT TG
WNK4 S: CAC CTC CCG CCG CAA CAG NM_175579 (rat) 347 This study
AS: TCC ACA CAG CAA AGA GCA CCC

Primers designed on mouse sequences also specifically recognized and amplified the corresponding rat genes. All primer sets were validated to specifically recognize their targets.

Pondugula et al.

Pondugula et al. BMC Physiology 2013 13:6   doi:10.1186/1472-6793-13-6

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