Table 1

RT-PCR demonstration of the presence of KCC1, KCC3a, KCC3b, KCC4 and absence of KCC2 in rat SCCD primary cultures
Ct, average
 Tissue 18S KCC1 KCC2 KCC3a KCC3b KCC4
rSCCD 26.4 BT 25.0 27.3 26.7
rat kidney 14.2 25.9 -- 23.3 24.5 26.3
rat brain -- -- 22.6 -- -- --
blank 35.0 30.2 BT BT 32.3 35.8

BT, Below Threshold fluorescence at end of 38 thermocycles. Ct values are based on duplicate runs of 4 samples SCCD and duplicates of control tissue total RNA. The difference between Ct values of duplicates varied by 0.3 ± 0.04 (n=26) with a range from 0.0 to 0.8.

Pondugula et al.

Pondugula et al. BMC Physiology 2013 13:6   doi:10.1186/1472-6793-13-6

Open Data