Open Access Open Badges Research article

The antioxidant response of the liver of male Swiss mice raised on a AIN 93 or commercial diet

Aline C Caetano1, Lucimara F da Veiga2, Flávia R Capaldi3, Severino M de Alencar2, Ricardo A Azevedo3 and Rosangela MN Bezerra4*

Author Affiliations

1 Postgraduate Program in Food Science and Technology, Escola Superior de Agricultura “Luiz de Queiroz”, University of São Paulo (USP), Piracicaba, SP, Brazil

2 Department of Agri- Food Industry, Food and Nutrition, “Luiz de Queiroz” College of Agriculture, University of São Paulo (USP), Piracicaba, SP, Brazil

3 Department of Genetics, “Luiz de Queiroz” College of Agriculture, University of São Paulo (USP), Piracicaba, SP, Brazil

4 School of Applied Sciences, University of Campinas (UNICAMP), 1300, Pedro Zaccaria St, Jd Sta Luiza, 13484-350, Limeira, São Paulo, Brazil

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BMC Physiology 2013, 13:3  doi:10.1186/1472-6793-13-3

Published: 24 January 2013



Reactive oxygen species (ROS) are formed under natural physiological conditions and are thought to play an important role in many human diseases. A wide range of antioxidants are involved in cellular defense mechanisms against ROS, which can be generated in excess during stressful conditions, these include enzymes and non-enzymatic antioxidants. The aim of this study was to evaluate the antioxidant responses of mice to two diets control, commercial and the purified AIN 93 diet, commonly used in experiments with rodents.


Malondialdehyde (MDA) and hydrogen peroxide (H2O2) concentrations and superoxide dismutase (SOD) and glutathione reductase (GR) activities determined in the liver were lower in the group of mice fed with the AIN 93 diet, while catalase (CAT) activity was higher in the same group, when compared to the group fed on the commercial diet. Liver glutathione peroxidase (GSH-Px) activity was similar in the groups fed on either AIN 93 or the commercial diets. Two SOD isoforms, Mn-SODII and a Cu/Zn-SODV, were specifically reduced in the liver of the AIN 93 diet fed animals.


The clear differences in antioxidant responses observed in the livers of mice fed on the two diets suggest that the macro- and micro-nutrient components with antioxidant properties, including vitamin E, can promote changes in the activity of enzymes involved in the removal of the ROS generated by cell metabolism.

AIN 93 diet; Antioxidant enzymes; Commercial diet; Lipid peroxidation; Liver; Mice; Oxidative stress; Reactive oxygen species