Table 2

List of primers used for the qPCR reactions
Genes Short name Sequence 5' to 3' Accession
radical S-adenosyl methionine domain-containing protein 21 Rsad2 F-GTACCGCAGATGCACAACAC AF076620
R-TTGACACTGCTTGGAGTTGC
interferon inducible protein Gig11 Gig1 F-GGCAACCTGAATCCAGAAGA DW569595
R-GTCTGGACGCAGACTGATGA
VHSV-inducible protein1 Vhsv2 F-GGTGAAGACCTGGACCTGAA BT072288
R-TGACCCCTGTTGACCTTCTC
interferon-induced protein with tetratricopeptide repeats 51 Ifit5 F-CAGAGAGGTGCCAGGCTAAC BT046021
R-TGCACATTGACTCTCCTTGG
interferon induced with helicase C domain 11 Mda5 F-CAGAGGTGGGGTTCAATGAT NM001195179
R-AGCTCGCTCCACTTGTTGAT
DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide RIG-I1 Rig-I F-GACGGTCAGCAGGGTGTACT DY714827
R-CCCGTGTCCTAACGAACAGT
barrier-to-autointegration factor1 Baf F-ACAGACCCCTCATCATCCTG BT049316
R-CGGTGCTTTTGAGAAGTGGT
signal transducer and activator of transcription 1 isoform alpha1 Stat1a F-CGGTGGAGCCCTACACTAAG CB513054
R-GGGATCCTGGGGTAGAGGTA
interferon-inducible protein Gig2-like1 Gig2 F-GATGTTTCATGGCTGCTCAA BT044026
R-CTTTTCGGATGTCCCGACTA
B-type natriuretic peptide2 BNP F-TCGACAAATCCGCAATAAGA CK883650
R-TTGAGCCAATTCGGTCTAGC
putative collagen alpha 12 put_Coll-a1 F-AACCCTGAACCCCTCAGTCT CA038317
R-TGGTCCTACCGTCTGGTTTC
leukocyte elastase inhibitor2 LEI F-TCTCAGATGGCAAAGGCTCT BT045959
R-GTTGGCCAGTTTCAGGATGT
elongation factor 1a3 EF1α F-CACCACGGGCCATCTGATCTACAA BT072490
R-TCAGCAGCCTCCTTCTCGAACTTC
18S rRNA3 18S F-GCCCTATCAACTTTCGATGGTAC AJ427629
R-TTTGGATGTGGTAGCCGTTTCTC
infectious pancreatic necrosis virus_polyprotein4 IPNV F-CCGACCGAGAACAT AJ877117
R-TGACAGCTTGACCCTGGTGAT

1 Virus-responsive genes (Figure 4).

2 Genes used for microarray validation.

3 Reference genes used for normalization.

4 IPNV gene used for relative virus quantification (described in Results section).

Castro et al.

Castro et al. BMC Physiology 2013 13:1   doi:10.1186/1472-6793-13-1

Open Data