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Open Access Highly Accessed Research article

Cell proliferation along vascular islands during microvascular network growth

Molly R Kelly-Goss1, Erica R Winterer1, Peter C Stapor1, Ming Yang1, Richard S Sweat1, William B Stallcup2, Geert W Schmid-Schönbein3 and Walter L Murfee1*

Author affiliations

1 Department of Biomedical Engineering, Tulane University, New Orleans, LA, 70118, USA

2 Sanford-Burnham Medical Research Institute, La Jolla, CA, 92037, USA

3 Department of Bioengineering, University of California-San Diego, LA Jolla, CA, 92093-0412, USA

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Citation and License

BMC Physiology 2012, 12:7  doi:10.1186/1472-6793-12-7

Published: 21 June 2012

Abstract

Background

Observations in our laboratory provide evidence of vascular islands, defined as disconnected endothelial cell segments, in the adult microcirculation. The objective of this study was to determine if vascular islands are involved in angiogenesis during microvascular network growth.

Results

Mesenteric tissues, which allow visualization of entire microvascular networks at a single cell level, were harvested from unstimulated adult male Wistar rats and Wistar rats 3 and 10 days post angiogenesis stimulation by mast cell degranulation with compound 48/80. Tissues were immunolabeled for PECAM and BRDU. Identification of vessel lumens via injection of FITC-dextran confirmed that endothelial cell segments were disconnected from nearby patent networks. Stimulated networks displayed increases in vascular area, length density, and capillary sprouting. On day 3, the percentage of islands with at least one BRDU-positive cell increased compared to the unstimulated level and was equal to the percentage of capillary sprouts with at least one BRDU-positive cell. At day 10, the number of vascular islands per vascular area dramatically decreased compared to unstimulated and day 3 levels.

Conclusions

These results show that vascular islands have the ability to proliferate and suggest that they are able to incorporate into the microcirculation during the initial stages of microvascular network growth.

Keywords:
Angiogenesis; Microcirculation; Mesentery; Proliferation; Endothelial cell