Chronic BCG instillation induces a predominant migration of myeloperoxidase-positive neutrophils and, to a lesser extent, F4/80-positive macrophages that are blunted by co-treatment with VEGF neutralizing antibody (B20). Quantification of myeloperoxidase-IR (A-B) and F4/80-IR (C-D) in the sub urothelium and detrusor muscle of bladders isolated from: control (mice receiving i.p. PBS [twice a week] concomitant to 4 weekly instillations of PBS), BCG-treated (mice receiving i.p. PBS [twice a week] concomitant to 4 weekly instillations of BCG), and B20-treated (mice receiving i.p. B20 [5 mg/kg; twice a week] concomitant to 4 weekly instillations of BCG). N = 8 per group. Statistical significance was set for p values < 0.05.
Saban et al. BMC Physiology 2011 11:16 doi:10.1186/1472-6793-11-16