Table 1 |
||||
|
Details of primer sequences used in qPCR analysis |
||||
|
Gene of interest |
Accesion numbers |
Sequence of primers |
Product size |
Annealing temp (°C) |
|
|
||||
|
COL1A2 |
5' GGCACTCCAGGTCCTCAG 3' 5' CCACAGCACCAGCAACAC 3' |
100 |
60 |
|
|
ALP |
5' GCTGAACAGGAACAACGTGA3' 5' TCAATTCTGCCTCCTTCCAC 3' |
117 |
60 |
|
|
OPG |
5'GCAGCGGCACATTGGACATG 5'AGGATCTGGTCACTGGGTTTGC3' |
135 |
60 |
|
|
β-ACTIN |
5' GGACCTGACTGACTACCTC 3' 5' GCCATCTCTTGCTCGAAG 3' |
135 |
60 |
|
|
GIPr |
5' GACCAAAGGCTCATCTTGGA 3' 5' ATGTAGCCGCCTGAACAAAC 3' |
114 |
60 |
|
|
GLP-1r |
5' TGGACCAGGAACTCCAACAT 3' 5' TTTGGATACCACGATGCAGA 3' |
114 |
62.5 |
|
|
GLP-2r |
5' TTCCTTTATTGGGCGTTCA 3' 5' CTCTCCATTGGCAAAACCA 3' |
155 |
60 |
|
|
GHSr 1a |
5' GCACTCTTCGTGGTGGGCAT 3' 5' GATGAGCAGATCGGAGAAGG 3' |
123 |
60.5 |
|
|
GPR39 |
5' GCTCATGAAAAGCCAGAAGG 3' 5' CATGATCCTCCGAATCTGGT 3' |
172 |
60 |
|
|
|
||||
|
Pacheco-Pantoja et al. BMC Physiology 2011 11:12 doi:10.1186/1472-6793-11-12 |
||||
