Osteocalcin levels after treatments with gut hormones in MG-63 osteoblastic cell lines. Cells were serum deprived and treated for 48 or 120 h with the gut peptides at indicated molar concentrations. Straight lines solid triangle markers designate 48 h treatment; dashed lines empty triangle markers indicate 120 h treatment. Only GLP-2 (C) caused significant changes in OC production in MG-63, with increased secretion levels after short exposure, and decreased production after longer exposure. Results are the average of percentage of change in relation to controls; n = 5. ± SEM.
Pacheco-Pantoja et al. BMC Physiology 2011 11:12 doi:10.1186/1472-6793-11-12