COL1A2, ALP, OPG and gut hormone expression profiles. After extraction and reverse transcription, mRNA levels were analysed. Results are expressed as means of at least six replicates per cell line ±SEM. β-actin was used as the housekeeping gene to normalize the levels of mRNA expression. In all the cases these determinations showed highest expression in Saos-2 and the lowest rate was for MG-63. Water was used as non-template control. COL1A2, ALP and OPG had their highest expression in Saos-2, and the lowest in MG-63. GIP-R, GHS-R, and GPR39 mRNA expression was found in the three osteoblastic cell lines. GLP-1R and GLP-2R expression was observed only in MG-63 and TE-85.
Pacheco-Pantoja et al. BMC Physiology 2011 11:12 doi:10.1186/1472-6793-11-12