Table 3

Effect of daf-1 6 RNAi on pvit-2:gfp expression in daf-2(e1370) adult hermaphrodites

Reporter

Line 1^

Line 2^

Line 3^

Line 4^


pvit-2(2.0):gfp

68%(0, 1)

37%(0.1, 5)

30%(0.2, 5)

35%(0, 1)


pvit-2(1.5):gfp

47%(0.04, 2)


pvit-2(0.8):gfp

26% (0.2, 5)*

48%(0.2, 2)


pvit-2(0.3):gfp

30%(0, 1)

40%(0, 1)

24%(0, 1)


pgly-19:gfp

-3% (0, 1)

-28%(0, 1)


^ Relative GFP fluorescence (SD, trials). Data show % change (SD, trials) of average maximum GFP fluorescence from daf-16 RNAi versus control in daf-2(e1370) hermaphrodites. Positive values indicate increased pvit-2:GFP fluorescence associated with reduced daf-16 activity from RNAi. Bold indicates GFP intensity was significantly different in daf-16 RNAi-treated animals in all trials (p < 0.05, T-test).

* GFP fluorescence intensity increased statistically significantly in 3/5 trials (p ≤ 0.05, T-test). In the remaining 2 trials, % GFP intensity change on daf-16 RNA was +5% (p = 0.3) and +6% (p = 0.6)

DePina et al. BMC Physiology 2011 11:11   doi:10.1186/1472-6793-11-11

Open Data