Figure 2.

Suppression of vitellogenesis in daf-2(e1370) hermaphrodites. A) vit-2 mRNA levels relative to day 1 wildtype hermaphrodites in daf-2(e1368), daf-2(e1370) and daf-16(mgDf50); daf-2(e1370) adult hermaphrodites. (Left) vit-2 mRNA levels were similar to wildtype in daf-2(e1368). (Center) In daf-2(1370) hermaphrodites, vit-2 mRNA levels were similar to wildtype on days 1-4, but were significantly lower on day 6 (p = 0.001, T-test). (Right) The reduction of vit-2 mRNA in daf-2(e1370) was daf-16-dependent and was suppressed in daf-16(mgDf50); daf-2(e1370) hermaphrodites. See Table 1 for statistical summary. B) Direct comparison of vit-2 mRNA levels in day 6 adults of indicated genotype. Error bars, SEM; ** p < 0.001, T-test vs. wildtype day 6. C) sod-3 mRNA levels in daf-2(e1370) hermaphrodites. There was no statistically significant change between days 1-6 (p = 0.78, ANOVA). D) Coomassie-stained SDS-PAGE analysis of total lysates for measuring YP levels. E) Quantification of YP170 and actin abundance relative to myosin, as indicated. In day 1 daf-2(e1370) hermaphrodites, YP170 levels were reduced compared to wildtype and daf-16(mgDf50); daf-2(e1370) hermaphrodites (p < 0.01, T-test). In addition, YP170 increased in day 4 wildtype and daf-16(mgDf50); daf-2(e1370) hermaphrodites, but remained low in daf-2(e1370) hermaphrodites (p < 0.01, e1370 vs wildtype, day 4). There were no significant differences in actin levels, relative to myosin, between strains.

DePina et al. BMC Physiology 2011 11:11   doi:10.1186/1472-6793-11-11
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