Open Access Research article

Peristalsis in the junction region of the Drosophila larval midgut is modulated by DH31 expressing enteroendocrine cells

Dennis R LaJeunesse*, Brooke Johnson, Jason S Presnell, Kathleen Kay Catignas and Grzegorz Zapotoczny

Author Affiliations

Department of Biology, 312 Eberhart Bldg., University of North Carolina Greensboro, Greensboro, North Carolina 27402, USA

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BMC Physiology 2010, 10:14  doi:10.1186/1472-6793-10-14

Published: 10 August 2010

Additional files

Additional file 1:

Animation of an individual Midgut Junction DH31 expressing cell. This animation depicts a single Midgut Junction DH31 expressing cell within the wall of the midgut at the junction region. The apical end is noted by ruffled edge which extends into the lumen of the gut.

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Additional file 2:

Attachment of the muscular tether of the gastric caeca to the Midgut Junction region. In this animation of a z-series taken through an intact intestine shows the juxtaposition of a gastric caeca (marked with an arrow) and the midgut junction region, note the presence of GFP expressing Midgut Junction DH31 expressing cells. Higher levels of the DLG protein can be seen in the muscular tether when compared to the remainder of the gut.

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Additional file 3:

Rotation of the Gastric Caeca with muscular tether attached. In this animation the muscular tether extends from the tip of the gastric caeca (marked with an arrow).

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Additional file 4:

Remnant of the muscular tether insertion at the midgut junction region. In this animation, the connection between the muscle tether and the midgut has been severed leaving only the origin site of the tether in the midgut junction region. The muscle tether branches out and appears to insinuate into the visceral musculature of the midgut junction region which is marked in green by the presence of Midgut Junction DH31 expressing cells.

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Additional file 5:

Supplementary Table 1, Ablation of Midgut Junction DH31 expressing cells in the anterior midgut does not alter the morphology of the anterior midgut junction region. A table showing no change in the overall size of the midgut junction region when the Dh31 expressing cells are ablated.

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Additional file 6:

Supplementary Table 2, Ablation of Midgut Junction DH31 expressing cells does not change speed that food moves through gut. A table showing the results from a pulse-chase feeding experiment showing no change in the overall movement of food through the gut when the Dh31 expressing cells are ablated from the midgut junction region.

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Additional file 7:

Midgut Peristalsis at a wild type junction region. The movie shows the peristalsis within the anterior midgut junction region over the course of 50 seconds (total run time ~1.5 minute). The movie has been looped three times, and the rate increased to 4 frames per second from 2. The region is noted by the remains of the muscular tethers which are projecting from the midgut to the left. Six peristaltic contractions can be observed during this time in this region of the gut.

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Additional file 8:

Peristalsis in a Midgut Junction region missing the Midgut Junction DH31 expressing cells. The movie shows the peristalsis within the anterior midgut junction region over the course of 50 seconds (total run time ~1.5 minute). The movie has been looped three times, and the rate increased to 4 frames per second from 2. The anterior midgut junction region is noted by the remains of the muscular tethers which come into view when the midgut rotates during play. In this example, the Midgut Junction DH31 expressing cells were ablated by expression of UASricin via the DJ752 Gal4 driver. Although there are no contractions during this period, the midgut does rotate due to gut motility further down the intestine.

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Additional file 9:

Midgut Peristalsis in the Midgut from larvae homozygous for Dh31KG09001 . The movie shows the peristalsis within the anterior midgut junction region over the course of 60 seconds (total run time ~1.5 minute). The movie has been looped three times, and the rate increased to 4 frames per second from 2. The anterior midgut junction region is noted by the remains of the muscular tethers which come into view when the midgut rotates during play. As with Additional File 8, there is little motility in this region of the midgut when DH31 is not present.

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