Open Access Methodology article

Capturing chloroplast variation for molecular ecology studies: a simple next generation sequencing approach applied to a rainforest tree

Hannah McPherson12*, Marlien van der Merwe12, Sven K Delaney3, Mark A Edwards4, Robert J Henry5, Emma McIntosh1, Paul D Rymer16, Melita L Milner1, Juelian Siow1 and Maurizio Rossetto1

Author Affiliations

1 National Herbarium of NSW, The Royal Botanic Gardens and Domain Trust, Mrs Macquaries Road, 2000, Sydney, NSW, Australia

2 Australian Centre for Evolutionary Biology and Biodiversity, School of Earth and Environmental Science, University of Adelaide, Adelaide, SA, Australia

3 School of Biotechnology and Biomolecular Sciences, University of New South Wales, 2052, Sydney, NSW, Australia

4 Southern Cross Plant Science, Southern Cross University, 2480, Lismore, NSW, Australia

5 Queensland Alliance for Agriculture and Food Innovation, University of Queensland, 4072, Brisbane, QLD, Australia

6 Hawkesbury Institute for the Environment, University of Western Sydney, Hawkesbury Campus, 2753, Richmond, NSW, Australia

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BMC Ecology 2013, 13:8  doi:10.1186/1472-6785-13-8

Published: 14 March 2013

Additional files

Additional file 1:

Toona ciliata samples used for this study.

Format: XLS Size: 26KB Download file

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Additional file 2:

PCR primers designed for SNP detection, assessment of differences between Illumina and 454 sequencing and verification of inverted repeat flanking regions in Toona ciliata.

Format: XLS Size: 34KB Download file

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Open Data