Correlation analyses of environmental barcoding using different sources of DNA and primer sets as shown in number of 454 sequence reads matching each taxon from reference Sanger library. Graphs are represented below diagonal squares. In each graph number of 454 reads are plotted for two different 454 experiments comparing sources of DNA (ethanol-based DNA versus tissue-DNA) and primer sets used. Diagonal squares represent labels for X and Y-axis in columns and rows. For example, the first graph represents data from ethanol-based DNA using primer set AD in X-axis and tissue-DNA using primer set AD in Y-axis. R2 values for each graph are represented above diagonal squares and correspond to mirroring graphs below diagonal squares. For example, the analysis of ethanol-based DNA versus tissue-DNA in primer set AD is supported by an R2 value of 1. Font size corresponds to higher R2 values.
Hajibabaei et al. BMC Ecology 2012 12:28 doi:10.1186/1472-6785-12-28