Open Access Highly Accessed Research article

Behavioral mechanisms and morphological symptoms of zombie ants dying from fungal infection

David P Hughes12*, Sandra B Andersen2, Nigel L Hywel-Jones3, Winanda Himaman4, Johan Billen5 and Jacobus J Boomsma2

Author Affiliations

1 Departments of Entomology and Biology, Penn State University, PA 16802, USA

2 Centre for Social Evolution, Department of Biology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen, Denmark

3 Mycology Laboratory, National Center for Genetic Engineering and Biotechnology, Science Park, Pathum Thani 12120, Thailand

4 Forest Entomology and Microbiology Group, National Park, Department of National Parks, Wildlife and Plant Conservation, Bangkok 10900, Thailand

5 Zoological Institute, University of Leuven, Naamsestraat 59, 3000 Leuven, Belgium

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BMC Ecology 2011, 11:13  doi:10.1186/1472-6785-11-13

Published: 9 May 2011

Abstract

Background

Parasites that manipulate host behavior can provide prominent examples of extended phenotypes: parasite genomes controlling host behavior. Here we focus on one of the most dramatic examples of behavioral manipulation, the death grip of ants infected by Ophiocordyceps fungi. We studied the interaction between O. unilateralis s.l. and its host ant Camponotus leonardi in a Thai rainforest, where infected ants descend from their canopy nests down to understory vegetation to bite into abaxial leaf veins before dying. Host mortality is concentrated in patches (graveyards) where ants die on sapling leaves ca. 25 cm above the soil surface where conditions for parasite development are optimal. Here we address whether the sequence of ant behaviors leading to the final death grip can also be interpreted as parasite adaptations and describe some of the morphological changes inside the heads of infected workers that mediate the expression of the death grip phenotype.

Results

We found that infected ants behave as zombies and display predictable stereotypical behaviors of random rather than directional walking, and of repeated convulsions that make them fall down and thus precludes returning to the canopy. Transitions from erratic wandering to death grips on a leaf vein were abrupt and synchronized around solar noon. We show that the mandibles of ants penetrate deeply into vein tissue and that this is accompanied by extensive atrophy of the mandibular muscles. This lock-jaw means the ant will remain attached to the leaf after death. We further present histological data to show that a high density of single celled stages of the parasite within the head capsule of dying ants are likely to be responsible for this muscular atrophy.

Conclusions

Extended phenotypes in ants induced by fungal infections are a complex example of behavioral manipulation requiring coordinated changes of host behavior and morphology. Future work should address the genetic basis of such extended phenotypes.

Keywords:
extended phenotype; behavioral manipulation; ants; fungi; convergent evolution; parasites